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Infect. Immun. doi:10.1128/IAI.00669-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A Candida albicans mannoprotein deprived of its mannan moiety is efficiently taken up, processed by human dendritic cells and induces T cell activation without stimulating proinflammatory cytokine production

Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, 06126 Perugia, Italy; and Department of Infectious, Parasitic and immune-Mediated Diseases, Istituto Superiore di Sanità, 00186 Rome, Italy

^* To whom correspondence should be addressed. Email: vecchiar{at}unipg.it.

	Abstract

Mannoproteins are cell wall components of pathogenic fungi playing major virulence and immunogenic roles with both their mannan and protein moieties. The 65 kilodalton mannoprotein (MP65) of Candida albicans is a -glucanase adhesin recognized as a major target of the human immune response against this fungus, and its recombinant product (rMP65 – devoid of the mannan moiety) is presently under consideration as a vaccine candidate. Here we investigated cellular and molecular aspects of the interaction of rMP65 with human antigen presenting cells. We also assessed the ability of rMP65 to initiate a T cell response. Both the native mannosylated MP65 (nMP65) and the recombinant product were efficiently bound and taken up by macrophages and dendritic cells. However, contrarily to nMP65, rMP65 did not induce TNF- and IL-6 release from these cells. On the other hand, rMP65 was rapidly endocytosed by both macrophages and dendritic cells in a process involving both clathrin-dependent and clathrin-independent mechanisms. Moreover, the RGD sequence inhibits to some extent rMP65 uptake. After internalization, rMP65 partially co-localized with lysosomal membrane-associated glycoproteins 1 and 2. This possibly resulted into efficient protein degradation and presentation to CD4⁺ T cells, which proliferated and produced Interferon-. Collectively, these results demonstrate that absence of the mannan moiety does not deprive MP65 of the capacity to initiate the pattern of cellular and molecular events leading to antigen presentation and T cell activation, which are essential features for further consideration of MP65 as a potential vaccine candidate.