This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sorensen, A. L.
Right arrow Articles by Andersen, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sorensen, A. L.
Right arrow Articles by Andersen, A. B.

 Previous Article  |  Next Article 

Infect. Immun., 05 1995, 1710-1717, Vol 63, No. 5
Copyright © 1995, American Society for Microbiology

Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis

AL Sorensen, S Nagai, G Houen, P Andersen and AB Andersen
Mycobacteria Department, Statens Seruminstitut, Copenhagen, Denmark.

A novel immunogenic antigen, the 6-kDa early secretory antigenic target (ESAT-6), from short-term culture filtrates of Mycobacterium tuberculosis was purified by hydrophobic interaction chromatography and anion-exchange chromatography by use of fast protein liquid chromatography. The antigen focused at two different pIs of 4.0 and 4.5 during isoelectric focusing, and each of these components separated into three spots ranging from 4 to 6 kDa during two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The apparent differences in molecular masses or pIs of these isoforms were not due to posttranslational glycosylation. The molecular weight of the purified native protein was determined by applying gel filtration and nondenaturing polyacrylamide gel electrophoresis and found to be 24 kDa. ESAT-6 is recognized by the murine monoclonal antibody HYB 76-8, which was used to screen a recombinant lambda gt11 M. tuberculosis DNA library. A phage expressing a gene product recognized by HYB 76-8 was isolated, and a 1.7-kbp fragment of the mycobacterial DNA insert was sequenced. The structural gene of ESAT-6 was identified as the sequence encoding a polypeptide of 95 amino acids. The N terminus of the deduced sequence could be aligned with the 10 amino-terminal amino acids derived from sequence analyses of the native protein. N-terminal sequence analysis showed that the purified antigen was essentially free from contaminants, and the amino acid analysis of the antigen was in good agreement with the DNA sequence-deduced amino acid composition. Thus, the heterogeneities observed in the pI and molecular weight of the purified antigen do not derive from contaminating proteins but are most likely due to heterogeneity of the antigen itself. Native and recombinant ESAT-6 are immunologically active in that both elicited a high release of gamma interferon from T cells isolated from memory- immune mice challenged with M. tuberculosis. Analyses of subcellular fractions of M. tuberculosis showed the presence of ESAT-6 in cytosol- and cell wall-containing fractions. Interspecies analyses showed the presence of ESAT-6 in filtrates from M. tuberculosis complex species. Among filtrates from mycobacteria not belonging to the M. tuberculosis complex, reactivity was observed in Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum.


This article has been cited by other articles:

  • Hoefsloot, W., van Ingen, J., de Lange, W. C. M., Dekhuijzen, P. N. R., Boeree, M. J., van Soolingen, D. (2009). Clinical relevance of Mycobacterium malmoense isolation in the Netherlands. Eur Respir J 34: 926-931 [Abstract] [Full Text]  
  • van Ingen, J., de Zwaan, R., Dekhuijzen, R., Boeree, M., van Soolingen, D. (2009). Region of Difference 1 in Nontuberculous Mycobacterium Species Adds a Phylogenetic and Taxonomical Character. J. Bacteriol. 191: 5865-5867 [Abstract] [Full Text]  
  • Thacker, T. C., Palmer, M. V., Waters, W. R. (2009). T-Cell mRNA Expression in Response to Mycobacterium bovis BCG Vaccination and Mycobacterium bovis Infection of White-Tailed Deer. CVI 16: 1139-1145 [Abstract] [Full Text]  
  • Leversen, N. A., de Souza, G. A., Malen, H., Prasad, S., Jonassen, I., Wiker, H. G. (2009). Evaluation of signal peptide prediction algorithms for identification of mycobacterial signal peptides using sequence data from proteomic methods. Microbiology 155: 2375-2383 [Abstract] [Full Text]  
  • Wang, X., Barnes, P. F., Dobos-Elder, K. M., Townsend, J. C., Chung, Y.-t., Shams, H., Weis, S. E., Samten, B. (2009). ESAT-6 Inhibits Production of IFN-{gamma} by Mycobacterium tuberculosis-Responsive Human T Cells. J. Immunol. 182: 3668-3677 [Abstract] [Full Text]  
  • Shin, A-R., Shin, S. J., Lee, K.-S., Eom, S.-H., Lee, S.-S., Lee, B.-S., Lee, J.-S., Cho, S. N., Kim, H.-J. (2008). Improved Sensitivity of Diagnosis of Tuberculosis in Patients in Korea via a Cocktail Enzyme-Linked Immunosorbent Assay Containing the Abundantly Expressed Antigens of the K Strain of Mycobacterium tuberculosis. CVI 15: 1788-1795 [Abstract] [Full Text]  
  • Meintjes, G., Wilkinson, K. A., Rangaka, M. X., Skolimowska, K., van Veen, K., Abrahams, M., Seldon, R., Pepper, D. J., Rebe, K., Mouton, P., van Cutsem, G., Nicol, M. P., Maartens, G., Wilkinson, R. J. (2008). Type 1 Helper T Cells and FoxP3-positive T Cells in HIV-Tuberculosis-associated Immune Reconstitution Inflammatory Syndrome. Am. J. Respir. Crit. Care Med. 178: 1083-1089 [Abstract] [Full Text]  
  • Garufi, G., Butler, E., Missiakas, D. (2008). ESAT-6-Like Protein Secretion in Bacillus anthracis. J. Bacteriol. 190: 7004-7011 [Abstract] [Full Text]  
  • Harrington, N. P., Surujballi, O. P., Prescott, J. F., Duncan, J. R., Waters, W. R., Lyashchenko, K., Greenwald, R. (2008). Antibody Responses of Cervids (Cervus elaphus) following Experimental Mycobacterium bovis Infection and the Implications for Immunodiagnosis. CVI 15: 1650-1658 [Abstract] [Full Text]  
  • Gallegos, A. M., Pamer, E. G., Glickman, M. S. (2008). Delayed protection by ESAT-6-specific effector CD4+ T cells after airborne M. tuberculosis infection. JEM 205: 2359-2368 [Abstract] [Full Text]  
  • Sidders, B., Pirson, C., Hogarth, P. J., Hewinson, R. G., Stoker, N. G., Vordermeier, H. M., Ewer, K. (2008). Screening of Highly Expressed Mycobacterial Genes Identifies Rv3615c as a Useful Differential Diagnostic Antigen for the Mycobacterium tuberculosis Complex. Infect. Immun. 76: 3932-3939 [Abstract] [Full Text]  
  • Reiley, W. W., Calayag, M. D., Wittmer, S. T., Huntington, J. L., Pearl, J. E., Fountain, J. J., Martino, C. A., Roberts, A. D., Cooper, A. M., Winslow, G. M., Woodland, D. L. (2008). ESAT-6-specific CD4 T cell responses to aerosol Mycobacterium tuberculosis infection are initiated in the mediastinal lymph nodes. Proc. Natl. Acad. Sci. USA 105: 10961-10966 [Abstract] [Full Text]  
  • Lightbody, K. L., Ilghari, D., Waters, L. C., Carey, G., Bailey, M. A., Williamson, R. A., Renshaw, P. S., Carr, M. D. (2008). Molecular Features Governing the Stability and Specificity of Functional Complex Formation by Mycobacterium tuberculosis CFP-10/ESAT-6 Family Proteins. J. Biol. Chem. 283: 17681-17690 [Abstract] [Full Text]  
  • Golby, P., Nunez, J., Cockle, P. J., Ewer, K., Logan, K., Hogarth, P., Vordermeier, H. M., Hinds, J., Hewinson, R. G., Gordon, S. V. (2008). Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology 154: 1059-1067 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiol. Mol. Biol. Rev. 71: 495-548 [Abstract] [Full Text]  
  • Gibbons, H. S., Wolschendorf, F., Abshire, M., Niederweis, M., Braunstein, M. (2007). Identification of Two Mycobacterium smegmatis Lipoproteins Exported by a SecA2-Dependent Pathway. J. Bacteriol. 189: 5090-5100 [Abstract] [Full Text]  
  • Sibbald, M. J. J. B., Ziebandt, A. K., Engelmann, S., Hecker, M., de Jong, A., Harmsen, H. J. M., Raangs, G. C., Stokroos, I., Arends, J. P., Dubois, J. Y. F., van Dijl, J. M. (2006). Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics. Microbiol. Mol. Biol. Rev. 70: 755-788 [Abstract] [Full Text]  
  • Sereinig, S., Stukova, M., Zabolotnyh, N., Ferko, B., Kittel, C., Romanova, J., Vinogradova, T., Katinger, H., Kiselev, O., Egorov, A. (2006). Influenza Virus NS Vectors Expressing the Mycobacterium tuberculosis ESAT-6 Protein Induce CD4+ Th1 Immune Response and Protect Animals against Tuberculosis Challenge.. CVI 13: 898-904 [Abstract] [Full Text]  
  • Mustafa, A. S., Skeiky, Y. A., Al-Attiyah, R., Alderson, M. R., Hewinson, R. G., Vordermeier, H. M. (2006). Immunogenicity of Mycobacterium tuberculosis Antigens in Mycobacterium bovis BCG-Vaccinated and M. bovis-Infected Cattle.. Infect. Immun. 74: 4566-4572 [Abstract] [Full Text]  
  • Codecasa, L., Mantegani, P., Galli, L., Lazzarin, A., Scarpellini, P., Fortis, C. (2006). An In-House RD1-Based Enzyme-Linked Immunospot-Gamma Interferon Assay Instead of the Tuberculin Skin Test for Diagnosis of Latent Mycobacterium tuberculosis Infection.. J. Clin. Microbiol. 44: 1944-1950 [Abstract] [Full Text]  
  • Demissie, A., Leyten, E. M. S., Abebe, M., Wassie, L., Aseffa, A., Abate, G., Fletcher, H., Owiafe, P., Hill, P. C., Brookes, R., Rook, G., Zumla, A., Arend, S. M., Klein, M., Ottenhoff, T. H. M., Andersen, P., Doherty, T. M., the VACSEL Study Group, (2006). Recognition of Stage-Specific Mycobacterial Antigens Differentiates between Acute and Latent Infections with Mycobacterium tuberculosis. CVI 13: 179-186 [Abstract] [Full Text]  
  • Delgado, J. C., Baena, A., Thim, S., Goldfeld, A. E. (2006). Aspartic Acid Homozygosity at Codon 57 of HLA-DQ {beta} Is Associated with Susceptibility to Pulmonary Tuberculosis in Cambodia. J. Immunol. 176: 1090-1097 [Abstract] [Full Text]  
  • Brodin, P., Majlessi, L., Marsollier, L., de Jonge, M. I., Bottai, D., Demangel, C., Hinds, J., Neyrolles, O., Butcher, P. D., Leclerc, C., Cole, S. T., Brosch, R. (2006). Dissection of ESAT-6 System 1 of Mycobacterium tuberculosis and Impact on Immunogenicity and Virulence. Infect. Immun. 74: 88-98 [Abstract] [Full Text]  
  • Goletti, D., Vincenti, D., Carrara, S., Butera, O., Bizzoni, F., Bernardini, G., Amicosante, M., Girardi, E. (2005). Selected RD1 Peptides for Active Tuberculosis Diagnosis: Comparison of a Gamma Interferon Whole-Blood Enzyme-Linked Immunosorbent Assay and an Enzyme-Linked Immunospot Assay. CVI 12: 1311-1316 [Abstract] [Full Text]  
  • Doherty, T. M., Andersen, P. (2005). Vaccines for Tuberculosis: Novel Concepts and Recent Progress. Clin. Microbiol. Rev. 18: 687-702 [Abstract] [Full Text]  
  • Davidow, A., Kanaujia, G. V., Shi, L., Kaviar, J., Guo, X., Sung, N., Kaplan, G., Menzies, D., Gennaro, M. L. (2005). Antibody Profiles Characteristic of Mycobacterium tuberculosis Infection State. Infect. Immun. 73: 6846-6851 [Abstract] [Full Text]  
  • Ko, H.-J., Ko, S.-Y., Kim, Y.-J., Lee, E.-G., Cho, S.-N., Kang, C.-Y. (2005). Optimization of Codon Usage Enhances the Immunogenicity of a DNA Vaccine Encoding Mycobacterial Antigen Ag85B. Infect. Immun. 73: 5666-5674 [Abstract] [Full Text]  
  • Koo, H. C., Park, Y. H., Ahn, J., Waters, W. R., Palmer, M. V., Hamilton, M. J., Barrington, G., Mosaad, A. A., Park, K. T., Jung, W. K., Hwang, I. Y., Cho, S.-N., Shin, S. J., Davis, W. C. (2005). Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis. J. Clin. Microbiol. 43: 4498-4506 [Abstract] [Full Text]  
  • Fortune, S. M., Jaeger, A., Sarracino, D. A., Chase, M. R., Sassetti, C. M., Sherman, D. R., Bloom, B. R., Rubin, E. J. (2005). Mutually dependent secretion of proteins required for mycobacterial virulence. Proc. Natl. Acad. Sci. USA 102: 10676-10681 [Abstract] [Full Text]  
  • Matsumoto, S., Matsumoto, M., Umemori, K., Ozeki, Y., Furugen, M., Tatsuo, T., Hirayama, Y., Yamamoto, S., Yamada, T., Kobayashi, K. (2005). DNA Augments Antigenicity of Mycobacterial DNA-Binding Protein 1 and Confers Protection against Mycobacterium tuberculosis Infection in Mice. J. Immunol. 175: 441-449 [Abstract] [Full Text]  
  • Wilkinson, K. A., Simsova, M., Scholvinck, E., Sebo, P., Leclerc, C., Vordermeier, H. M., Dickson, S. J., Brown, J. R., Davidson, R. N., Pasvol, G., Levin, M., Wilkinson, R. J. (2005). Efficient Ex Vivo Stimulation of Mycobacterium tuberculosis-Specific T Cells by Genetically Detoxified Bordetella pertussis Adenylate Cyclase Antigen Toxoids. Infect. Immun. 73: 2991-2998 [Abstract] [Full Text]  
  • Ravn, P., Munk, M. E., Andersen, A. B., Lundgren, B., Lundgren, J. D., Nielsen, L. N., Kok-Jensen, A., Andersen, P., Weldingh, K. (2005). Prospective Evaluation of a Whole-Blood Test Using Mycobacterium tuberculosis-Specific Antigens ESAT-6 and CFP-10 for Diagnosis of Active Tuberculosis. CVI 12: 491-496 [Abstract] [Full Text]  
  • Demangel, C., Garnier, T., Rosenkrands, I., Cole, S. T. (2005). Differential Effects of Prior Exposure to Environmental Mycobacteria on Vaccination with Mycobacterium bovis BCG or a Recombinant BCG Strain Expressing RD1 Antigens. Infect. Immun. 73: 2190-2196 [Abstract] [Full Text]  
  • Pheiffer, C., Betts, J. C., Flynn, H. R., Lukey, P. T., van Helden, P. (2005). Protein expression by a Beijing strain differs from that of another clinical isolate and Mycobacterium tuberculosis H37Rv. Microbiology 151: 1139-1150 [Abstract] [Full Text]  
  • Majlessi, L., Brodin, P., Brosch, R., Rojas, M.-J., Khun, H., Huerre, M., Cole, S. T., Leclerc, C. (2005). Influence of ESAT-6 Secretion System 1 (RD1) of Mycobacterium tuberculosis on the Interaction between Mycobacteria and the Host Immune System. J. Immunol. 174: 3570-3579 [Abstract] [Full Text]  
  • Converse, S. E., Cox, J. S. (2005). A Protein Secretion Pathway Critical for Mycobacterium tuberculosis Virulence Is Conserved and Functional in Mycobacterium smegmatis. J. Bacteriol. 187: 1238-1245 [Abstract] [Full Text]  
  • Tully, G., Kortsik, C., Hohn, H., Zehbe, I., Hitzler, W. E., Neukirch, C., Freitag, K., Kayser, K., Maeurer, M. J. (2005). Highly Focused T Cell Responses in Latent Human Pulmonary Mycobacterium tuberculosis Infection. J. Immunol. 174: 2174-2184 [Abstract] [Full Text]  
  • Burts, M. L., Williams, W. A., DeBord, K., Missiakas, D. M. (2005). EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc. Natl. Acad. Sci. USA 102: 1169-1174 [Abstract] [Full Text]  
  • Weldingh, K., Rosenkrands, I., Okkels, L. M., Doherty, T. M., Andersen, P. (2005). Assessing the Serodiagnostic Potential of 35 Mycobacterium tuberculosis Proteins and Identification of Four Novel Serological Antigens. J. Clin. Microbiol. 43: 57-65 [Abstract] [Full Text]  
  • Kamath, A. B., Woodworth, J., Xiong, X., Taylor, C., Weng, Y., Behar, S. M. (2004). Cytolytic CD8+ T Cells Recognizing CFP10 Are Recruited to the Lung after Mycobacterium tuberculosis Infection. JEM 200: 1479-1489 [Abstract] [Full Text]  
  • Koo, H. C., Park, Y. H., Ahn, J., Waters, W. R., Hamilton, M. J., Barrington, G., Mosaad, A. A., Palmer, M. V., Shin, S., Davis, W. C. (2004). New Latex Bead Agglutination Assay for Differential Diagnosis of Cattle Infected with Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis. CVI 11: 1070-1074 [Abstract] [Full Text]  
  • Aoki, K., Matsumoto, S., Hirayama, Y., Wada, T., Ozeki, Y., Niki, M., Domenech, P., Umemori, K., Yamamoto, S., Mineda, A., Matsumoto, M., Kobayashi, K. (2004). Extracellular Mycobacterial DNA-binding Protein 1 Participates in Mycobacterium-Lung Epithelial Cell Interaction through Hyaluronic Acid. J. Biol. Chem. 279: 39798-39806 [Abstract] [Full Text]  
  • Scarpellini, P., Tasca, S., Galli, L., Beretta, A., Lazzarin, A., Fortis, C. (2004). Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection. J. Clin. Microbiol. 42: 3469-3474 [Abstract] [Full Text]  
  • Spencer, J. S., Kim, H. J., Marques, A. M., Gonzalez-Juarerro, M., Lima, M. C. B. S., Vissa, V. D., Truman, R. W., Gennaro, M. L., Cho, S.-N., Cole, S. T., Brennan, P. J. (2004). Comparative Analysis of B- and T-Cell Epitopes of Mycobacterium leprae and Mycobacterium tuberculosis Culture Filtrate Protein 10. Infect. Immun. 72: 3161-3170 [Abstract] [Full Text]  
  • Brock, I., Weldingh, K., Leyten, E. M. S., Arend, S. M., Ravn, P., Andersen, P. (2004). Specific T-Cell Epitopes for Immunoassay-Based Diagnosis of Mycobacterium tuberculosis Infection. J. Clin. Microbiol. 42: 2379-2387 [Abstract] [Full Text]  
  • Tjalsma, H., Antelmann, H., Jongbloed, J. D.H., Braun, P. G., Darmon, E., Dorenbos, R., Dubois, J.-Y. F., Westers, H., Zanen, G., Quax, W. J., Kuipers, O. P., Bron, S., Hecker, M., van Dijl, J. M. (2004). Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome. Microbiol. Mol. Biol. Rev. 68: 207-233 [Abstract] [Full Text]  
  • Mehta, R., Pearson, J. T., Mahajan, S., Nath, A., Hickey, M. J., Sherman, D. R., Atkins, W. M. (2004). Adenylylation and Catalytic Properties of Mycobacterium tuberculosis Glutamine Synthetase Expressed in Escherichia coli versus Mycobacteria. J. Biol. Chem. 279: 22477-22482 [Abstract] [Full Text]  
  • Liu, X.-Q., Dosanjh, D., Varia, H., Ewer, K., Cockle, P., Pasvol, G., Lalvani, A. (2004). Evaluation of T-Cell Responses to Novel RD1- and RD2-Encoded Mycobacterium tuberculosis Gene Products for Specific Detection of Human Tuberculosis Infection. Infect. Immun. 72: 2574-2581 [Abstract] [Full Text]  
  • Okkels, L. M., Andersen, P. (2004). Protein-Protein Interactions of Proteins from the ESAT-6 Family of Mycobacterium tuberculosis. J. Bacteriol. 186: 2487-2491 [Abstract] [Full Text]  
  • Shi, L., North, R., Gennaro, M. L. (2004). Effect of Growth State on Transcription Levels of Genes Encoding Major Secreted Antigens of Mycobacterium tuberculosis in the Mouse Lung. Infect. Immun. 72: 2420-2424 [Abstract] [Full Text]  
  • Kanaujia, G. V., Motzel, S., Garcia, M. A., Andersen, P., Gennaro, M. L. (2004). Recognition of ESAT-6 Sequences by Antibodies in Sera of Tuberculous Nonhuman Primates. CVI 11: 222-226 [Abstract] [Full Text]  
  • Huard, R. C., Chitale, S., Leung, M., Lazzarini, L. C. O., Zhu, H., Shashkina, E., Laal, S., Conde, M. B., Kritski, A. L., Belisle, J. T., Kreiswirth, B. N., Lapa e Silva, J. R., Ho, J. L. (2003). The Mycobacterium tuberculosis Complex-Restricted Gene cfp32 Encodes an Expressed Protein That Is Detectable in Tuberculosis Patients and Is Positively Correlated with Pulmonary Interleukin-10. Infect. Immun. 71: 6871-6883 [Abstract] [Full Text]  
  • Okkels, L. M., Brock, I., Follmann, F., Agger, E. M., Arend, S. M., Ottenhoff, T. H. M., Oftung, F., Rosenkrands, I., Andersen, P. (2003). PPE Protein (Rv3873) from DNA Segment RD1 of Mycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family. Infect. Immun. 71: 6116-6123 [Abstract] [Full Text]  
  • Kohler, A. K., Stone, D. M., Hines, M. T., Byrne, B. A., Alperin, D. C., Norton, L. K., Hines, S. A. (2003). Rhodococcus equi Secreted Antigens Are Immunogenic and Stimulate a Type 1 Recall Response in the Lungs of Horses Immune to R. equi Infection. Infect. Immun. 71: 6329-6337 [Abstract] [Full Text]  
  • Hsu, T., Hingley-Wilson, S. M., Chen, B., Chen, M., Dai, A. Z., Morin, P. M., Marks, C. B., Padiyar, J., Goulding, C., Gingery, M., Eisenberg, D., Russell, R. G., Derrick, S. C., Collins, F. M., Morris, S. L., King, C. H., Jacobs, W. R. Jr. (2003). The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue. Proc. Natl. Acad. Sci. USA 100: 12420-12425 [Abstract] [Full Text]  
  • Yeremeev, V. V., Kondratieva, T. K., Rubakova, E. I., Petrovskaya, S. N., Kazarian, K. A., Telkov, M. V., Biketov, S. F., Kaprelyants, A. S., Apt, A. S. (2003). Proteins of the Rpf Family: Immune Cell Reactivity and Vaccination Efficacy against Tuberculosis in Mice. Infect. Immun. 71: 4789-4794 [Abstract] [Full Text]  
  • Aagaard, C., Govaerts, M., Meng Okkels, L., Andersen, P., Pollock, J. M. (2003). Genomic Approach to Identification of Mycobacterium bovis Diagnostic Antigens in Cattle. J. Clin. Microbiol. 41: 3719-3728 [Abstract] [Full Text]  
  • Garnier, T., Eiglmeier, K., Camus, J.-C., Medina, N., Mansoor, H., Pryor, M., Duthoy, S., Grondin, S., Lacroix, C., Monsempe, C., Simon, S., Harris, B., Atkin, R., Doggett, J., Mayes, R., Keating, L., Wheeler, P. R., Parkhill, J., Barrell, B. G., Cole, S. T., Gordon, S. V., Hewinson, R. G. (2003). The complete genome sequence of Mycobacterium bovis. Proc. Natl. Acad. Sci. USA 100: 7877-7882 [Abstract] [Full Text]  
  • Bao, L., Chen, W., Zhang, H., Wang, X. (2003). Virulence, Immunogenicity, and Protective Efficacy of Two Recombinant Mycobacterium bovis Bacillus Calmette-Guerin Strains Expressing the Antigen ESAT-6 from Mycobacterium tuberculosis. Infect. Immun. 71: 1656-1661 [Abstract] [Full Text]  
  • Latchumanan, V. K., Singh, B., Sharma, P., Natarajan, K. (2002). Mycobacterium tuberculosis Antigens Induce the Differentiation of Dendritic Cells from Bone Marrow. J. Immunol. 169: 6856-6864 [Abstract] [Full Text]  
  • Cardoso, F. L. L., Antas, P. R. Z., Milagres, A. S., Geluk, A., Franken, K. L. M. C., Oliveira, E. B., Teixeira, H. C., Nogueira, S. A., Sarno, E. N., Klatser, P., Ottenhoff, T. H. M., Sampaio, E. P. (2002). T-Cell Responses to the Mycobacterium tuberculosis-Specific Antigen ESAT-6 in Brazilian Tuberculosis Patients. Infect. Immun. 70: 6707-6714 [Abstract] [Full Text]  
  • Haile, Y., Bjune, G., Wiker, H. G. (2002). Expression of the mceA, esat-6 and hspX genes in Mycobacterium tuberculosis and their responses to aerobic conditions and to restricted oxygen supply. Microbiology 148: 3881-3886 [Abstract] [Full Text]  
  • Gey van Pittius, N. C., Warren, R. M., van Helden, P. D. (2002). ESAT-6 and CFP-10: What Is the Diagnosis?. Infect. Immun. 70: 6509-6511 [Full Text]  
  • Geluk, A., Ottenhoff, T. H. M., Arend, S. M., Andersen, P., Doherty, T. M. (2002). Authors' Reply. Infect. Immun. 70: 6509-6511 [Full Text]  
  • Skjot, R. L. V., Brock, I., Arend, S. M., Munk, M. E., Theisen, M., Ottenhoff, T. H. M., Andersen, P. (2002). Epitope Mapping of the Immunodominant Antigen TB10.4 and the Two Homologous Proteins TB10.3 and TB12.9, Which Constitute a Subfamily of the esat-6 Gene Family. Infect. Immun. 70: 5446-5453 [Abstract] [Full Text]  
  • Cole, S. T. (2002). Comparative and functional genomics of the Mycobacterium tuberculosis complex. Microbiology 148: 2919-2928 [Full Text]  
  • Renshaw, P. S., Panagiotidou, P., Whelan, A., Gordon, S. V., Hewinson, R. G., Williamson, R. A., Carr, M. D. (2002). Conclusive Evidence That the Major T-cell Antigens of the Mycobacterium tuberculosis Complex ESAT-6 and CFP-10 Form a Tight, 1:1 Complex and Characterization of the Structural Properties of ESAT-6, CFP-10, and the ESAT-6{middle dot}CFP-10 Complex. IMPLICATIONS FOR PATHOGENESIS AND VIRULENCE. J. Biol. Chem. 277: 21598-21603 [Abstract] [Full Text]  
  • Flyer, D. C., Ramakrishna, V., Miller, C., Myers, H., McDaniel, M., Root, K., Flournoy, C., Engelhard, V. H., Canaday, D. H., Marto, J. A., Ross, M. M., Hunt, D. F., Shabanowitz, J., White, F. M. (2002). Identification by Mass Spectrometry of CD8+-T-Cell Mycobacterium tuberculosis Epitopes within the Rv0341 Gene Product. Infect. Immun. 70: 2926-2932 [Abstract] [Full Text]  
  • Vordermeier, H. M., Chambers, M. A., Cockle, P. J., Whelan, A. O., Simmons, J., Hewinson, R. G. (2002). Correlation of ESAT-6-Specific Gamma Interferon Production with Pathology in Cattle following Mycobacterium bovis BCG Vaccination against Experimental Bovine Tuberculosis. Infect. Immun. 70: 3026-3032 [Abstract] [Full Text]  
  • Geluk, A., van Meijgaarden, K. E., Franken, K. L. M. C., Subronto, Y. W., Wieles, B., Arend, S. M., Sampaio, E. P., de Boer, T., Faber, W. R., Naafs, B., Ottenhoff, T. H. M. (2002). Identification and Characterization of the ESAT-6 Homologue of Mycobacterium leprae and T-Cell Cross-Reactivity with Mycobacterium tuberculosis. Infect. Immun. 70: 2544-2548 [Abstract] [Full Text]  
  • Spencer, J. S., Marques, M. A. M., Lima, M. C. B. S., Junqueira-Kipnis, A. P., Gregory, B. C., Truman, R. W., Brennan, P. J. (2002). Antigenic Specificity of the Mycobacterium leprae Homologue of ESAT-6. Infect. Immun. 70: 1010-1013 [Abstract] [Full Text]  
  • Recchi, C., Rauzier, J., Gicquel, B., Reyrat, J.-M. (2002). Signal-sequence-independent secretion of the staphylococcal nuclease in Mycobacterium smegmatis. Microbiology 148: 529-536 [Abstract] [Full Text]  
  • Cappelli, G., Volpe, P., Sanduzzi, A., Sacchi, A., Colizzi, V., Mariani, F. (2001). Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication of Mycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97. Infect. Immun. 69: 7262-7270 [Abstract] [Full Text]  
  • Arend, S. M., Engelhard, A. C. F., Groot, G., de Boer, K., Andersen, P., Ottenhoff, T. H. M., van Dissel, J. T. (2001). Tuberculin Skin Testing Compared with T-Cell Responses to Mycobacterium tuberculosis-Specific and Nonspecific Antigens for Detection of Latent Infection in Persons with Recent Tuberculosis Contact. CVI 8: 1089-1096 [Abstract] [Full Text]  
  • Olsen, A. W., van Pinxteren, L. A. H., Okkels, L. M., Rasmussen, P. B., Andersen, P. (2001). Protection of Mice with a Tuberculosis Subunit Vaccine Based on a Fusion Protein of Antigen 85B and ESAT-6. Infect. Immun. 69: 2773-2778 [Abstract] [Full Text]  
  • LALVANI, A., PATHAN, A. A., MCSHANE, H., WILKINSON, R. J., LATIF, M., CONLON, C. P., PASVOL, G., HILL, A. V. S. (2001). Rapid Detection of Mycobacterium tuberculosis Infection by Enumeration of Antigen-specific T Cells. Am. J. Respir. Crit. Care Med. 163: 824-828 [Abstract] [Full Text]  
  • Lewinsohn, D. M., Zhu, L., Madison, V. J., Dillon, D. C., Fling, S. P., Reed, S. G., Grabstein, K. H., Alderson, M. R. (2001). Classically Restricted Human CD8+ T Lymphocytes Derived from Mycobacterium tuberculosis-Infected Cells: Definition of Antigenic Specificity. J. Immunol. 166: 439-446 [Abstract] [Full Text]  
  • Betts, J. C., Dodson, P., Quan, S., Lewis, A. P., Thomas, P. J., Duncan, K., McAdam, R. A. (2000). Comparison of the proteome of Mycobacterium tuberculosis strain H37Rv with clinical isolate CDC 1551. Microbiology 146: 3205-3216 [Abstract] [Full Text]  
  • Dockrell, H. M., Brahmbhatt, S., Robertson, B. D., Britton, S., Fruth, U., Gebre, N., Hunegnaw, M., Hussain, R., Manandhar, R., Murillo, L., Pessolani, M. C. V., Roche, P., Salgado, J. L., Sampaio, E., Shahid, F., Thole, J. E. R., Young, D. B. (2000). A Postgenomic Approach to Identification of Mycobacterium leprae-Specific Peptides as T-Cell Reagents. Infect. Immun. 68: 5846-5855 [Abstract] [Full Text]  
  • Ulrichs, T., Anding, P., Porcelli, S., Kaufmann, S. H. E., Munk, M. E. (2000). Increased Numbers of ESAT-6- and Purified Protein Derivative-Specific Gamma Interferon-Producing Cells in Subclinical and Active Tuberculosis Infection. Infect. Immun. 68: 6073-6076 [Abstract] [Full Text]  
  • Dillon, D. C., Alderson, M. R., Day, C. H., Bement, T., Campos-Neto, A., Skeiky, Y. A. W., Vedvick, T., Badaro, R., Reed, S. G., Houghton, R. (2000). Molecular and Immunological Characterization of Mycobacterium tuberculosis CFP-10, an Immunodiagnostic Antigen Missing in Mycobacterium bovis BCG. J. Clin. Microbiol. 38: 3285-3290 [Abstract] [Full Text]  
  • Arend, S. M., Geluk, A., van Meijgaarden, K. E., van Dissel, J. T., Theisen, M., Andersen, P., Ottenhoff, T. H. M. (2000). Antigenic Equivalence of Human T-Cell Responses to Mycobacterium tuberculosis-Specific RD1-Encoded Protein Antigens ESAT-6 and Culture Filtrate Protein 10 and to Mixtures of Synthetic Peptides. Infect. Immun. 68: 3314-3321 [Abstract] [Full Text]  
  • Rhodes, S. G., Gavier-Widen, D., Buddle, B. M., Whelan, A. O., Singh, M., Hewinson, R. G., Vordermeier, H. M. (2000). Antigen Specificity in Experimental Bovine Tuberculosis. Infect. Immun. 68: 2573-2578 [Abstract] [Full Text]  
  • Musser, J. M., Amin, A., Ramaswamy, S. (2000). Negligible Genetic Diversity of Mycobacterium tuberculosis Host Immune System Protein Targets: Evidence of Limited Selective Pressure. Genetics 155: 7-16 [Abstract] [Full Text]  
  • Gomez, M., Johnson, S., Gennaro, M. L. (2000). Identification of Secreted Proteins of Mycobacterium tuberculosis by a Bioinformatic Approach. Infect. Immun. 68: 2323-2327 [Abstract] [Full Text]  
  • van Pinxteren, L. A. H., Ravn, P., Agger, E. M., Pollock, J., Andersen, P. (2000). Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10. CVI 7: 155-160 [Abstract] [Full Text]  
  • Alderson, M. R., Bement, T., Day, C. H., Zhu, L., Molesh, D., Skeiky, Y. A. W., Coler, R., Lewinsohn, D. M., Reed, S. G., Dillon, D. C. (2000). Expression Cloning of an Immunodominant Family of Mycobacterium tuberculosis Antigens Using Human Cd4+ T Cells. JEM 191: 551-560 [Abstract] [Full Text]  
  • Colangeli, R., Spencer, J. S., Bifani, P., Williams, A., Lyashchenko, K., Keen, M. A., Hill, P. J., Belisle, J., Gennaro, M. L. (2000). MTSA-10, the Product of the Rv3874 Gene of Mycobacterium tuberculosis, Elicits Tuberculosis-Specific, Delayed-Type Hypersensitivity in Guinea Pigs. Infect. Immun. 68: 990-993 [Abstract] [Full Text]  
  • Skjot, R. L. V., Oettinger, T., Rosenkrands, I., Ravn, P., Brock, I., Jacobsen, S., Andersen, P. (2000). Comparative Evaluation of Low-Molecular-Mass Proteins from Mycobacterium tuberculosis Identifies Members of the ESAT-6 Family as Immunodominant T-Cell Antigens. Infect. Immun. 68: 214-220 [Abstract] [Full Text]  
  • Johnson, P. D. R., Stuart, R. L., Grayson, M. L., Olden, D., Clancy, A., Ravn, P., Andersen, P., Britton, W. J., Rothel, J. S. (1999). Tuberculin-Purified Protein Derivative-, MPT-64-, and ESAT-6-Stimulated Gamma Interferon Responses in Medical Students before and after Mycobacterium bovis BCG Vaccination and in Patients with Tuberculosis. CVI 6: 934-937 [Abstract] [Full Text]  
  • Rosenkrands, I., Weldingh, K., Ravn, P., Brandt, L., Hojrup, P., Rasmussen, P. B., Coates, A. R., Singh, M., Mascagni, P., Andersen, P. (1999). Differential T-Cell Recognition of Native and Recombinant Mycobacterium tuberculosis GroES. Infect. Immun. 67: 5552-5558 [Abstract] [Full Text]  
  • Demissie, A., Ravn, P., Olobo, J., Doherty, T. M., Eguale, T., Geletu, M., Hailu, W., Andersen, P., Britton, S. (1999). T-Cell Recognition of Mycobacterium tuberculosis Culture Filtrate Fractions in Tuberculosis Patients and Their Household Contacts. Infect. Immun. 67: 5967-5971 [Abstract] [Full Text]  
  • Skeiky, Y. A. W., Lodes, M. J., Guderian, J. A., Mohamath, R., Bement, T., Alderson, M. R., Reed, S. G. (1999). Cloning, Expression, and Immunological Evaluation of Two Putative Secreted Serine Protease Antigens of Mycobacterium tuberculosis. Infect. Immun. 67: 3998-4007 [Abstract] [Full Text]  
  • Lyashchenko, K. P., Pollock, J. M., Colangeli, R., Gennaro, M. L. (1998). Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis. Infect. Immun. 66: 5344-5349 [Abstract] [Full Text]  
  • Coler, R. N., Skeiky, Y. A. W., Vedvick, T., Bement, T., Ovendale, P., Campos-Neto, A., Alderson, M. R., Reed, S. G. (1998). Molecular Cloning and Immunologic Reactivity of a Novel Low Molecular Mass Antigen of Mycobacterium tuberculosis. J. Immunol. 161: 2356-2364 [Abstract] [Full Text]  
  • Weldingh, K., Rosenkrands, I., Jacobsen, S., Rasmussen, P. B., Elhay, M. J., Andersen, P. (1998). Two-Dimensional Electrophoresis for Analysis of Mycobacterium tuberculosis Culture Filtrate and Purification and Characterization of Six Novel Proteins. Infect. Immun. 66: 3492-3500 [Abstract] [Full Text]  
  • Lyashchenko, K., Manca, C., Colangeli, R., Heijbel, A., Williams, A., Gennaro, M. L. (1998). Use of Mycobacterium tuberculosis Complex-Specific Antigen Cocktails for a Skin Test Specific for Tuberculosis. Infect. Immun. 66: 3606-3610 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»