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Cover photograph (Copyright © 2009, American Society for Microbiology. All Rights Reserved.): Burkholderia cenocepacia replicates in macrophages. Phorbol myristate acetate-treated U937 cells were infected with B. cenocepacia and incubated for 2 h. Extracellular bacteria were killed, and cells were incubated for another 16 h. Cells were fixed, and bacteria were visualized using AlexaFluor 594-detected antibody to B. cenocepacia. Cells were counterstained with DAPI (4′,6-diamidino-2-phenylindole). Confocal microscopy revealed large numbers of intracellular bacteria in the perinuclear area. In contrast, type IV secretion system mutant bacteria were often observed as degraded bacterial particles in the perinuclear or cytoplasmic area. (See related article in December 2008, vol. 76, p. 5447.)
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